Application of SPRi Biosensors for Determination of 20S Proteasome and UCH-L1 Levels in the Serum and Urine of Transitional Bladder Cancer Patients

The ubiquitin–proteasome system (UPS) participates in the degradation of proteins which play an important role regulating cell cycle, apoptosis, and angiogenesis, as well immune system. These processes are carcinogenesis. Transitional carcinoma (TCC) is one predominant types bladder cancer. relationship between cancer progression has become a topic increasing interest among researchers. In this work, we propose application surface plasmon resonance imaging (SPRi)-based biosensors for detection 20S proteasome ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) blood serum urine patients with TCC. aim study was to determine UCH-L1 concentrations correlate results clinicopathological parameters. group subjects consisted 82 confirmed TCC, addition control 27 healthy volunteers. It found that were significantly elevated both TCC patients, compared subjects. There correlation urine, concentration. SPRi biosensor sensitive using PSI inhibitor receptor, protein protein-specific antibody receptor suitable determination body fluids can serve useful tools investigation biomarkers.